Amyloid-beta 1-40 monoclonal antibody, clone BDI350
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More Files
- More Functions
Western Blot
Western blot analysis of Beta amyloid peptides were applied to SDS-PAGE and transferred to a PVDF membrane.
The blot was probed with 2 ug/mL Beta amyloid monoclonal antibody, clone BDI350 (Cat # MAB3913) for 1 hour at 15-22°C and developed by ECL.
Lane 1 : Beta amyloid (1-40).
Lane 2 : Beta amyloid (1-42).
Lane 3 : Beta amyloid (1-43).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Amyloid-beta 1-40.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human Amyloid-beta 1-40.
Host
Mouse
Reactivity
Human
Specificity
Specifically reacts with the C-terminus of human beta Amyloid 1-40. Does not cross-react with beta Amyloid 1-42.
Form
Lyophilized
Isotype
IgG1
Recommend Usage
ELISA (0.1 ug/mL)
Western Blot (1 ug/mL HRP/ECL detection)
Immunocytochemistry (1-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 2x PBS (PEG, sucrose, 0.09% sodium azide).
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of deionized water (15 minutes at room temperature), store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Beta amyloid peptides were applied to SDS-PAGE and transferred to a PVDF membrane.
The blot was probed with 2 ug/mL Beta amyloid monoclonal antibody, clone BDI350 (Cat # MAB3913) for 1 hour at 15-22°C and developed by ECL.
Lane 1 : Beta amyloid (1-40).
Lane 2 : Beta amyloid (1-42).
Lane 3 : Beta amyloid (1-43).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
Enzyme Immunoassay
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Gene Info — APP
Entrez GeneID
351Gene Name
APP
Gene Alias
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2
Gene Description
amyloid beta (A4) precursor protein
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq
Other Designations
A4 amyloid protein|amyloid beta A4 protein|amyloid-beta protein|beta-amyloid peptide|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II
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Interactome
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Disease
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