Mouse monoclonal antibody raised against 5-bromodeoxyuridine (BrdU).
5-bromodeoxyuridine (BrdU) conjugated with Hemocyanin.
This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. This antibody is also useful for detecting proliferating cells by flowcytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU).
Immunocytochemistry (2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (15 mM sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo ; paraffin-embedded sections) with 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (Cat # MAB3635).
Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM (human acute lymphoblastic leukemia cell line) using 5-bromodeoxyuridine (BrdU) monoclonal antibody, clone MoBu-1 (Cat # MAB3635) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.