ARAP1 monoclonal antibody, clone ARAP1-2
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More Files
- More Functions
Immunofluorescence
Colocalization of ARAP1 with DR4 at the human plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0', 30' or 60' and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1 monoclonal antibody, clone ARAP1-2 (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ARAP1.
Immunogen
Recombinant His6 fusion protein corresponding to amino acids 1190-1450 of human ARAP1.
Host
Mouse
Theoretical MW (kDa)
160
Reactivity
Human
Specificity
This antibody reacts with C-terminal part of ARAP1, a 160 KDa adaptor protein.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (2 ug/m)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
Colocalization of ARAP1 with DR4 at the human plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0', 30' or 60' and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1 monoclonal antibody, clone ARAP1-2 (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.Immunoprecipitation
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Gene Info — ARAP1
Entrez GeneID
116985Gene Name
ARAP1
Gene Alias
CENTD2, KIAA0782
Gene Description
ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Omim ID
606646Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1|centaurin, delta 2
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Interactome
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Pathway
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Publication Reference
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Comparison of effects of PKA catalytic subunit on I(h) and calcium channel currents in rat dorsal root ganglion cells.
Komagiri Y, Kitamura N.
Biomedical Research (Tokyo, Japan) 2007 Aug; 28(4):177.
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cAMP-independent role of PKA in tonicity-induced transactivation of tonicity-responsive enhancer/ osmotic response element-binding protein.
Ferraris JD, Persaud P, Williams CK, Chen Y, Burg MB.
PNAS 2002 Dec; 99(26):16800.
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Transgenic inhibitors identify two roles for protein kinase A in Drosophila development.
Kiger JA Jr, Eklund JL, Younger SH, O'Kane CJ.
Genetics 1999 May; 152(1):281.
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Comparison of effects of PKA catalytic subunit on I(h) and calcium channel currents in rat dorsal root ganglion cells.
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