Ptprc monoclonal antibody, clone RA3-6B2
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More Files
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Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Ptprc monoclonal antibody, clone RA3-6B2 (Cat # MAB3232)-R-phycoerythrin and rat anti-mouse Cd3e-FITC, clone C363.29B. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Ptprc.
Immunogen
Native Ptprc from abelson murine leukemia virus-induced pre-B tumor cells.
Host
Rat
Reactivity
Mouse
Specificity
B220 isoform of CD45, Mr 240 KDa.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Ptprc monoclonal antibody, clone RA3-6B2 (Cat # MAB3232)-R-phycoerythrin and rat anti-mouse Cd3e-FITC, clone C363.29B. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Ptprc
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Interactome
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Publication Reference
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Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells.
Watanabe Y, Akaike T.
Scandinavian Journal of Immunology 1994 May; 39(5):419.
Application：Flow Cyt, IP, Mouse , Mouse lymphocyte.
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In vivo treatment with anti B-220 monoclonal antibody affects T and B cell differentiation.
Asensi V, Himeno K, Kawamura I, Sakumoto M, Nomoto K.
Clinical and Experimental Immunology 1990 May; 80(2):268.
Application：Flow Cyt, Func, Mouse, Mouse spleen cells, Mouse thymus.
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Lymphokine-activated killer (LAK) cells. IV. Characterization of murine LAK effector subpopulations.
Ballas ZK, Rasmussen W.
Journal of Immunology 1990 Jan; 144(1):386.
Application：Flow Cyt, Mouse, Mouse splenocytes.
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Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells.
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