APAF1 monoclonal antibody, clone 2E10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of APAF1 in K-562 cell lysate with APAF1 monoclonal antibody, clone 2E10 (Cat # MAB2746) at (A) 0.25 and (B) 0.5 mg/ml.
Immunocytochemistry
Immunocytochemistry of APAF1 in K-562 cells with APAF1 monoclonal antibody, clone 2E10 (Cat # MAB2746) at 0.5 ug/mL .
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of APAF1.
Immunogen
A synthetic peptide corresponding to amino acids near C-terminus of human APAF1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG
Recommend Usage
Western Blot (0.25-0.5 mg/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of APAF1 in K-562 cell lysate with APAF1 monoclonal antibody, clone 2E10 (Cat # MAB2746) at (A) 0.25 and (B) 0.5 mg/ml.Immunohistochemistry
Immunocytochemistry
Immunocytochemistry of APAF1 in K-562 cells with APAF1 monoclonal antibody, clone 2E10 (Cat # MAB2746) at 0.5 ug/mL . -
Gene Info — APAF1
Entrez GeneID
317Protein Accession#
n/aGene Name
APAF1
Gene Alias
CED4, DKFZp781B1145
Gene Description
apoptotic peptidase activating factor 1
Omim ID
602233Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
apoptotic protease activating factor 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development.
Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P.
Cell 1998 Sep; 94(6):727.
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Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation.
Hu Y, Benedict MA, Wu D, Inohara N, Nunez G.
PNAS 1998 Apr; 95(8):4386.
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Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X.
Cell 1997 Nov; 91(4):479.
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Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development.
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