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Last updated: 2014/4/21
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BIOtech
BULK-new

SMAD1 monoclonal antibody, clone AF10B7

  • Catalog # : MAB2697
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant SMAD1.
  • Immunogen:
  • Recombinant His fusion protein corresponding to human SMAD1.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Rat
  • Specificity:
  • This antibody also react with SMAD5.
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • Western Blot (1:1000-2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In HEPES, 150 mM NaCl (50% glycerol, 0.01% BSA, 0.03% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblot analysis of cell lysates.
    Lane 1 : SK-N-MC cell lysate.
    Lnae 2 : Ramos cell lysate.
    Lane 3 : 293T cell lysate.
    Lane 4 : PC-12 cell lysate.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4086
  • Gene Name:
  • SMAD1
  • Gene Alias:
  • BSP1,JV4-1,JV41,MADH1,MADR1
  • Gene Description:
  • SMAD family member 1
  • Gene Summary:
  • The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
  • Other Designations:
  • MAD, mothers against decapentaplegic homolog 1,Mad-related protein 1,SMAD, mothers against DPP homolog 1,Sma- and Mad-related protein 1,TGF-beta signaling protein 1,mothers against DPP homolog 1,transforming growth factor-beta signaling protein 1
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