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Last updated: 2014/4/14
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BIOtech
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IRAK4 (phospho T345) monoclonal antibody, clone A8A8

  • Catalog # : MAB2538
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of IRAK4.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T345 of human IRAK4.
  • Sequence:
  • VMTSR
  • Host:
  • Mouse
  • Reactivity:
  • Bovine, Dog, Human, Mouse
  • Specificity:
  • This antibody recognizes IRAK4 (phospho T345) with a phosphorylated site at Threonine 345. It does not cross-react with non-phosphospecific peptide.
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The 5 x 107 Jurkat cells were stimulated by 50 ng/mL IL-1 for 10 min, collected, lysed, resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with Phosphospecific peptide. Lane 3 was pre-incubate with non-phospho specific peptide.An immunoreactive band is observed around ~51 KDa.
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptide was blot onto NC membrane.
    A : IRAK4 (pT345). B : IRAK4 (non phosphorylated). Followed by IRAK4 (phospho T345) monoclonal antibody, clone A8A8 (Cat # MAB2538) incubation at a 1 : 1000 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Gene Name:
  • IRAK4
  • Gene Alias:
  • IPD1,NY-REN-64,REN64
  • Gene Description:
  • interleukin-1 receptor-associated kinase 4
  • Gene Summary:
  • This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq
  • Other Designations:
  • IRAK-4 mutated form 1,interleukin-1 receptor associated kinase 4
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