CAV1 monoclonal antibody, clone 7C8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CAV1 in NIH/3T3 cell lysates (50 ug) using CAV1 monoclonal antibody, clone 7C8 (Cat # MAB2408). Lanes 1 and 2 : 1 : 4,000. Lanes 3 and 4 : 1 : 1,000. ECL : 5 minute exposure.
Immunofluorescence
Immunofluorescent imaging using CAV1 monoclonal antibody, clone 7C8 (Cat # MAB2408) at 1 : 200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane (Photo courtesy of Alberto Davalos, Yale School of Medicine).
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Specification
Product Description
Mouse monoclonal antibody raised against CAV1.
Immunogen
Glut 4 vesicles.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to caveolin alpha and beta proteins.
Form
Liquid
Isotype
IgG2b
Quality Control Testing
Antibody Reactive Against CAV1.
Recommend Usage
Immunofluorescence (1:200)
Western Blot (1:1000-1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CAV1 in NIH/3T3 cell lysates (50 ug) using CAV1 monoclonal antibody, clone 7C8 (Cat # MAB2408). Lanes 1 and 2 : 1 : 4,000. Lanes 3 and 4 : 1 : 1,000. ECL : 5 minute exposure.Immunofluorescence
Immunofluorescent imaging using CAV1 monoclonal antibody, clone 7C8 (Cat # MAB2408) at 1 : 200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane (Photo courtesy of Alberto Davalos, Yale School of Medicine).Immunoprecipitation
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Gene Info — CAV1
Entrez GeneID
857Gene Name
CAV1
Gene Alias
BSCL3, CAV, CGL3, MSTP085, VIP21
Gene Description
caveolin 1, caveolae protein, 22kDa
Omim ID
601047Gene Ontology
HyperlinkGene Summary
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq
Other Designations
OTTHUMP00000025031|caveolae protein, 22-kD|caveolin 1|caveolin 1 caveolae protein, 22kD|caveolin 1, alpha isoform|caveolin 1, beta isoform|caveolin-1 beta isoform protein|cell growth-inhibiting protein 32
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Interactome
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Pathway
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Disease
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Publication Reference
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Interferon-induced transmembrane protein 1-mediated EGFR/SOX2 signaling axis is essential for progression of non-small cell lung cancer.
Yang YG, Koh YW, Sari IN, Jun N, Lee S, Phi LTH, Kim KS, Wijaya YT, Lee SH, Baek MJ, Jeong D, Kwon HY.
International Journal of Cancer 2019 Apr; 144(8):2020.
Application：WB, Human, H1650 cells.
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Flotillin-1 facilitates toll-like receptor 3 signaling in human endothelial cells.
Fork C, Hitzel J, Nichols BJ, Tikkanen R, Brandes RP.
Basic Research in Cardiology 2014 Sep; 109(6):439.
Application：WB, Proximity ligation assay, Human, HUVECs.
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Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling.
Souto RP, Vallega G, Wharton J, Vinten J, Tranum-Jensen J, Pilch PF.
The Journal of Biological Chemistry 2003 May; 278(20):18321.
Application：WB-Ce, Immunoadsorption, Rat, Adipocytes, Beads.
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Interferon-induced transmembrane protein 1-mediated EGFR/SOX2 signaling axis is essential for progression of non-small cell lung cancer.
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