MGMT monoclonal antibody, clone MT 23.2
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant MGMT.
Immunogen
Recombinant protein corresponding to human MGMT.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to MGMT.
Form
Liquid
Isotype
IgG2b
Recommend Usage
Flow Cytometry (1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MGMT in CEM whole cell extract using MGMT monoclonal antibody, clone MT 23.2 (Cat # MAB2383) at 1 : 100 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Flow Cytometry
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Gene Info — MGMT
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Interactome
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Disease
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Publication Reference
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Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents.
Cai S, Xu Y, Cooper RJ, Ferkowicz MJ, Hartwell JR, Pollok KE, Kelley MR.
Cancer Research 2005 Apr; 65(8):3319.
Application:IF, WB-Ce, WB-Tr, Human, K562, HL-60, MDA231, SKOV-3, SKOV-3X, Hey, A204, HOS, LnCap, PC3, SJRH30 cells.
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O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma.
Zuo C, Ai L, Ratliff P, Suen JY, Hanna E, Brent TP, Fan CY.
Cancer Epidemiology, Biomarkers & Prevention 2004 Jun; 13(6):967.
Application:IHC-P, Human, Human head and neck squamous cell carcinoma.
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Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection.
Roth TJ, Xu Y, Luo M, Kelley MR.
Cancer Gene Therapy 2003 Aug; 10(8):603.
Application:WB, Human, K-562 cells.
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Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents.
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