FABP1 monoclonal antibody, clone 2G4
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: FABP1 Recombinant protein, Lane 2: HepG2 cell lysate, Lane 3: liver tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon cancer tissue were incubated with FABP1 monoclonal antibody, clone 2G4 (Cat # MAB2050) (1:100).
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with FABP1 monoclonal antibody, clone 2G4 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FABP1.
Immunogen
Recombinant protein corresponding to amino acids 1-127 of human FABP1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: FABP1 Recombinant protein, Lane 2: HepG2 cell lysate, Lane 3: liver tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon cancer tissue were incubated with FABP1 monoclonal antibody, clone 2G4 (Cat # MAB2050) (1:100).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with FABP1 monoclonal antibody, clone 2G4 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — FABP1
Entrez GeneID
2168Protein Accession#
NP_001434Gene Name
FABP1
Gene Alias
FABPL, L-FABP
Gene Description
fatty acid binding protein 1, liver
Omim ID
134650Gene Ontology
HyperlinkGene Summary
FABP1 encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP1 and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq
Other Designations
Fatty acid-binding protein, liver
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Interactome
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Pathway
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Disease
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Publication Reference
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Effect of pitavastatin on urinary liver-type fatty acid-binding protein levels in patients with early diabetic nephropathy.
Nakamura T, Sugaya T, Kawagoe Y, Ueda Y, Osada S, Koide H.
Diabetes Care 2005 Nov; 28(11):2728.
Application：ELISA, Human, Mouse, Urine, Recombinant protein.
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Liver fatty acid binding protein expression enhances branched-chain fatty acid metabolism.
Atshaves BP, Storey SM, Huang H, Schroeder F.
Molecular and Cellular Biochemistry 2004 Apr; 259(1-2):115.
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Effect of pitavastatin on urinary liver-type fatty acid-binding protein levels in patients with early diabetic nephropathy.
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