NANOG monoclonal antibody, clone 5A10
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NIH/3T3 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of of human seminoma tissue were incubated with NANOG monoclonal antibody, clone 5A10 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NANOG.
Immunogen
Recombinant protein corresponding to amino acids 1-154 of human NANOG.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of NIH/3T3 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of of human seminoma tissue were incubated with NANOG monoclonal antibody, clone 5A10 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — NANOG
-
Interactome
-
Publication Reference
-
MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation.
Tay Y, Zhang J, Thomson AM, Lim B, Rigoutsos I.
Nature 2008 Oct; 455(7216):1124.
Application：WB-Tr, Mouse, HEK 293T cells, Mouse embryonic stem cells.
-
Differential regulation of the Oct-3/4 gene in cell culture model systems that parallel different stages of mammalian development.
Mallanna SK, Boer B, Desler M, Rizzino A.
Molecular Reproduction and Development 2008 Aug; 75(8):1247.
-
Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma.
Chiou SH, Yu CC, Huang CY, Lin SC, Liu CJ, Tsai TH, Chou SH, Chien CS, Ku HH, Lo JF.
Clinical Cancer Research 2008 Jul; 14(13):4085.
Application：IF, WB, Human, Human oral squamous cell carcinoma, OC3, OECM1, SAS.
-
MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com