EPHA2 monoclonal antibody, clone 3D7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: EphA2 extracellular domain transfected 293T cell lysate, Lane 3: EphA2 cytoplasmic domain transfected 293T cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon (A) and gastric cancer tissue (B) were incubated with EPHA2 monoclonal antibody, clone 3D7 (Cat # MAB1769) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EPHA2.
Immunogen
Recombinant protein corresponding to amino acids 559-976 of human EPHA2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: EphA2 extracellular domain transfected 293T cell lysate, Lane 3: EphA2 cytoplasmic domain transfected 293T cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon (A) and gastric cancer tissue (B) were incubated with EPHA2 monoclonal antibody, clone 3D7 (Cat # MAB1769) (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPHA2
Entrez GeneID
1969Protein Accession#
P29317Gene Name
EPHA2
Gene Alias
ECK
Gene Description
EPH receptor A2
Omim ID
176946Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. [provided by RefSeq
Other Designations
ephrin receptor EphA2|epithelial cell receptor protein tyrosine kinase|protein tyrosine kinase|receptor protein tyrosine kinase regulated by p53 and E2F-1|soluble EPHA2 variant 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Locoregional delivery of CAR T cells to the cerebrospinal fluid for treatment of metastatic medulloblastoma and ependymoma.
Laura K Donovan, Alberto Delaidelli, Sujith K Joseph, Kevin Bielamowicz, Kristen Fousek, Borja L Holgado, Alex Manno, Dilakshan Srikanthan, Ahmed Z Gad, Randy Van Ommeren, David Przelicki, Cory Richman, Vijay Ramaswamy, Craig Daniels, Jonelle G Pallota, Tajana Douglas, Alyssa C M Joynt, Joonas Haapasalo, Carolina Nor, Maria C Vladoiu, Claudia M Kuzan-Fischer, Livia Garzia, Stephen C Mack, Srinidhi Varadharajan, Matthew L Baker, Liam Hendrikse, Michelle Ly, Kaitlin Kharas, Polina Balin, Xiaochong
Nature Medicine 2020 May; 26(5):720.
Application：IHC-P, Human, Human tissue microarrays.
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EphA2 receptor tyrosine kinase as a promising target for cancer therapeutics.
Ireton RC, Chen J.
Current Cancer Drug Targets 2005 May; 5(3):149.
Application：IHC-P, WB, Human, Breast cancer, Colon cancer, Prostate cancer, Lung cancer.
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Diverse roles for the Eph family of receptor tyrosine kinases in carcinogenesis.
Nakamoto M, Bergemann AD.
Microscopy Research and Technique 2002 Oct; 59(1):58.
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Locoregional delivery of CAR T cells to the cerebrospinal fluid for treatment of metastatic medulloblastoma and ependymoma.
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