CBL monoclonal antibody, clone M159
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of human Jurkat cells untreated (lane 1) or treated with pervanadate (1 mM) for 30 minutes (lane 2). The blot was probed with CBL monoclonal antibody, clone M159 (Cat # MAB1378).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of CBL.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CBL.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This sequence is highly conserved in rat and mouse c-Cbl.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of human Jurkat cells untreated (lane 1) or treated with pervanadate (1 mM) for 30 minutes (lane 2). The blot was probed with CBL monoclonal antibody, clone M159 (Cat # MAB1378).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CBL
Entrez GeneID
867Protein Accession#
P22681Gene Name
CBL
Gene Alias
C-CBL, CBL2, RNF55
Gene Description
Cas-Br-M (murine) ecotropic retroviral transforming sequence
Omim ID
165360Gene Ontology
HyperlinkGene Summary
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq
Other Designations
oncogene CBL2
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Interactome
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Pathway
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Disease
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Publication Reference
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Cbl: many adaptations to regulate protein tyrosine kinases.
Thien CB, Langdon WY.
Molecular and Cellular Biology 2001 Apr; 2(4):294.
Application：WB, Human, Mammalian cells.
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The protein product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the T cell antigen receptor.
Donovan JA, Wange RL, Langdon WY, Samelson LE.
The Journal of Biological Chemistry 1994 Sep; 269(37):22921.
Application：IP, WB-Ce, WB-Re, Human, Jurkat cells, Recombinant protein.
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Physical association between Src homology 3 elements and the protein product of the c-cbl proto-oncogene.
Rivero-Lezcano OM, Sameshima JH, Marcilla A, Robbins KC.
The Journal of Biological Chemistry 1994 Jul; 269(26):17363.
Application：IP, WB-Ce, Human, HL-60 cells.
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Cbl: many adaptations to regulate protein tyrosine kinases.
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