CCND1 monoclonal antibody, clone DCS-6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of C2C12, HepG2 and NIH3T3 cell lysates using CCND1 monoclonal antibody, clone DCS-6 (Cat # MAB11280) at 5 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human mantle cell lymphoma with CCND1 monoclonal antibody, clone DCS-6 (Cat # MAB11280) using peroxidase-conjugate and DAB chromogen.
Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min.
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Specification
Product Description
Mouse monoclonal antibody raised against full length CCND1.
Immunogen
Recombinant protein corresponding to full length human CCND1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G affinity chromatography
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (5-10 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.2 mg/ml PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of C2C12, HepG2 and NIH3T3 cell lysates using CCND1 monoclonal antibody, clone DCS-6 (Cat # MAB11280) at 5 ug/ml.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human mantle cell lymphoma with CCND1 monoclonal antibody, clone DCS-6 (Cat # MAB11280) using peroxidase-conjugate and DAB chromogen.
Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min. -
Gene Info — CCND1
Entrez GeneID
595Protein Accession#
NP_444284Gene Name
CCND1
Gene Alias
BCL1, D11S287E, PRAD1, U21B31
Gene Description
cyclin D1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq
Other Designations
B-cell CLL/lymphoma 1|G1/S-specific cyclin D1
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Interactome
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Pathway
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Disease
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