KLK3 monoclonal antibody, clone AT1D10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PC-3 cell lysate (40 ug) by using KLK3 monoclonal antibody, clone AT1D10 (Cat # MAB11194) (1:500-1:5000). Lane1 : 1:500. Lane2 : 1:1000. Lane 3 : 1:5000.
Immunofluorescence
Immunofluorescence analysis of KLK3 in LNCap cells. The cell was stained with KLK3 monoclonal antibody, clone AT1D10 (Cat # MAB11194) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of KLK3 in LNCaP cell line, staining at 2-5 ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KLK3.
Immunogen
Recombinant protein corresponding to amino acids 25-261 of human KLK3.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PC-3 cell lysate (40 ug) by using KLK3 monoclonal antibody, clone AT1D10 (Cat # MAB11194) (1:500-1:5000). Lane1 : 1:500. Lane2 : 1:1000. Lane 3 : 1:5000.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of KLK3 in LNCap cells. The cell was stained with KLK3 monoclonal antibody, clone AT1D10 (Cat # MAB11194) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of KLK3 in LNCaP cell line, staining at 2-5 ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — KLK3
Entrez GeneID
354Protein Accession#
P07288; NP_001639Gene Name
KLK3
Gene Alias
APS, KLK2A1, PSA, hK3
Gene Description
kallikrein-related peptidase 3
Omim ID
176820Gene Ontology
HyperlinkGene Summary
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
P-30 antigen|gamma-seminoprotein|kallikrein 3, (prostate specific antigen)|prostate specific antigen|semenogelase|seminin
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Interactome
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Pathway
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Disease
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