EPO monoclonal antibody, clone 4F11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) against HEK293 (1) and EPO-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence
Immunofluorescence analysis of GC-7901 cells using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EPO.
Immunogen
Recombinant protein corresponding to human EPO.
Host
Mouse
Theoretical MW (kDa)
21
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) against HEK293 (1) and EPO-hIgGFc transfected HEK293 (2) cell lysate.Immunofluorescence
Immunofluorescence analysis of GC-7901 cells using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPO
Entrez GeneID
2056Gene Name
EPO
Gene Alias
EP, MGC138142
Gene Description
erythropoietin
Omim ID
133170Gene Ontology
HyperlinkGene Summary
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq
Other Designations
epoetin
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Interactome
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Pathway
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Disease
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