EGF monoclonal antibody, clone 4E11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using EGF monoclonal antibody, clone 4E11 (Cat # MAB10631) against EGF-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using EGF monoclonal antibody, clone 4E11 (Cat # MAB10631) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EGF.
Immunogen
Recombinant protein corresponding to human EGF.
Host
Mouse
Theoretical MW (kDa)
134
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using EGF monoclonal antibody, clone 4E11 (Cat # MAB10631) against EGF-hIgGFc transfected HEK293 cell lysate.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using EGF monoclonal antibody, clone 4E11 (Cat # MAB10631) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGF
Entrez GeneID
1950Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactome
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Pathway
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Disease
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