CD9 monoclonal antibody, clone 5G6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542) against HEK293 (1) and CD9(aa : 37-228)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) and human kidney cancer(B) tissues using CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542).
Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of CD9.
Immunogen
A synthetic peptide corresponding to human CD9.
Host
Mouse
Theoretical MW (kDa)
25
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542) against HEK293 (1) and CD9(aa : 37-228)-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) and human kidney cancer(B) tissues using CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA detection with CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542).Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD9 monoclonal antibody, clone 5G6 (Cat # MAB10542) (green) and negative control (red). -
Gene Info — CD9
Entrez GeneID
928Gene Name
CD9
Gene Alias
5H9, BA2, BTCC-1, DRAP-27, GIG2, MIC3, MRP-1, P24, TSPAN29
Gene Description
CD9 molecule
Omim ID
143030Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. [provided by RefSeq
Other Designations
5H9 antigen|CD9 antigen|CD9 antigen (p24)|OTTHUMP00000041574|OTTHUMP00000041576|antigen defined by monoclonal antibody 602-29|growth-inhibiting gene 2 protein|leukocyte antigen MIC3|motility related protein|motility related protein-1|p24 antigen
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Interactome
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Pathway
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Disease
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