ATF2 monoclonal antibody, clone 4F12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using ATF2 monoclonal antibody, clone 4F12 (Cat # MAB10494) against NIH/3T3 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and brain tissues (B) using ATF2 monoclonal antibody, clone 4F12 (Cat # MAB10494) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with ATF2 monoclonal antibody, clone 4F12 (Cat # MAB10494).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ATF2.
Immunogen
Recombinant protein corresponding to human ATF2.
Host
Mouse
Theoretical MW (kDa)
55
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using ATF2 monoclonal antibody, clone 4F12 (Cat # MAB10494) against NIH/3T3 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and brain tissues (B) using ATF2 monoclonal antibody, clone 4F12 (Cat # MAB10494) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA detection with ATF2 monoclonal antibody, clone 4F12 (Cat # MAB10494). -
Gene Info — ATF2
Entrez GeneID
1386Gene Name
ATF2
Gene Alias
CRE-BP1, CREB2, HB16, MGC111558, TREB7
Gene Description
activating transcription factor 2
Omim ID
123811Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000163262|activating transcription factor 2 splice variant ATF2-var2|cAMP responsive element binding protein 2, formerly
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Interactome
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Pathway
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Disease
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