Mouse monoclonal antibody raised against native ITGAM.
Native purified ITGAM from human granulocytes.
Theoretical MW (kDa):
This antibody recognizes CD11b antigen (Mac-1), a 165 KDa type Itransmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (15 mM sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunoprecipitation of human CD11b / CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. Lysate was subjected to affinity column chromatography using ITGAM monoclonal antibody, clone MEM - 174 (Cat # MAB0877) immunosorbent. Eluted immunoprecipitate was resolved on 7.5 % SDS - PAGE and stained with Coomasie Blue.
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq