Products

Transfected Cell Line

HEK293

Plasmid

pCMV-HMGB1 full length

Host

Human

Theoretical MW (kDa)

25

Lysis Buffer

Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Concentration

2 mg/ml

Quality Control Testing

Transient overexpression cell lysate was tested with Anti-HMGB1 antibody (H00003146-M01) by Western Blots.

SDS-PAGE Gel

HMGB1 transfected lysate

Western Blot

Lane 1: HMGB1 transfected lysate ( 25 KDa).
Lane 2: Non-transfected lysate.

Recommend Usage

Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

Storage Buffer

In modified RIPA Lysis Buffer.

Storage Instruction

Store at -80°C. Aliquot to avoid repeated freezing and thawing.