EPB41 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-EPB41 full length
Host
Human
Theoretical MW (kDa)
81
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EPB41 antibody (H00002035-M01) by Western Blots.
SDS-PAGE Gel
EPB41 transfected lysate
Western Blot
Lane 1: EPB41 transfected lysate ( 81 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — EPB41
Entrez GeneID
2035GeneBank Accession#
BC039079Protein Accession#
AAH39079Gene Name
EPB41
Gene Alias
4.1R, EL1, HE
Gene Description
erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
Omim ID
130500Gene Ontology
HyperlinkGene Summary
Elliptocytosis is a hematologic disorder characterized by elliptically shaped erythrocytes and a variable degree of hemolytic anemia. Inherited as an autosomal dominant, elliptocytosis results from mutation in any one of several genes encoding proteins of the red cell membrane skeleton. The form discussed here is the one found in the 1950s to be linked to Rh blood group and more recently shown to be caused by a defect in protein 4.1. 'Rh-unlinked' forms of elliptocytosis are caused by mutation in the alpha-spectrin gene (MIM 182860), the beta-spectrin gene (MIM 182870), or the band 3 gene (MIM 109270).[supplied by OMIM
Other Designations
OTTHUMP00000003772|OTTHUMP00000003773|OTTHUMP00000003774|erythrocyte surface protein band 4.1
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