ACTB HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ACTB full length
Host
Human
Theoretical MW (kDa)
42
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACTB antibody (H00000060-M01) by Western Blots.
SDS-PAGE Gel
ACTB transfected lysate
Western Blot
Lane 1: ACTB transfected lysate ( 42 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — ACTB
Entrez GeneID
60GeneBank Accession#
BC001301Protein Accession#
AAH01301Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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