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BIOtech
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BMP7 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L003T6
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-BMP7 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 49.313
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-BMP7 antibody (H00000655-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L003T6
    BMP7 transfected lysate
    Western Blot
    QC Testing of L003T6
    Lane 1: BMP7 transfected lysate ( 49.313 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 655
  • Gene Name:
  • BMP7
  • Gene Alias:
  • OP-1
  • Gene Description:
  • bone morphogenetic protein 7
  • Gene Summary:
  • The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000031357,osteogenic protein 1
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