BMP7 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-BMP7 full length
Host
Human
Theoretical MW (kDa)
49.313
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BMP7 antibody (H00000655-M01) by Western Blots.
SDS-PAGE Gel
BMP7 transfected lysate
Western Blot
Lane 1: BMP7 transfected lysate ( 49.313 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — BMP7
Entrez GeneID
655GeneBank Accession#
BC008584Protein Accession#
AAH08584Gene Name
BMP7
Gene Alias
OP-1
Gene Description
bone morphogenetic protein 7
Omim ID
112267Gene Ontology
HyperlinkGene Summary
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq
Other Designations
OTTHUMP00000031357|osteogenic protein 1
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