A1CF purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Tissue lysate)
ACF MaxPab polyclonal antibody. Western Blot analysis of ACF expression in human placenta.
Western Blot (Cell lysate)
A1CF MaxPab polyclonal antibody. Western Blot analysis of A1CF expression in HeLa.
Western Blot (Transfected lysate)
Western Blot analysis of A1CF expression in transfected 293T cell line (H00029974-T02) by A1CF MaxPab polyclonal antibody.
Lane 1: ACF transfected lysate(65.34 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to A1CF on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human A1CF protein.
Immunogen
A1CF (NP_620311.1, 1 a.a. ~ 594 a.a) full-length human protein.
Sequence
MEAVCLGTCPEPEASMSTAIPGLKKGNNALQSIILQTLLEKENGQRKYGGPPPGWDAAPPERGCEIFIGKLPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIRNGRLLGVCASVDNCRLFVGGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPGRIQLWGHGIAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDYAFVHFSNREDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQVYDPTTTYLGAPVFYAPQTYAAIPSLHFPATKGHLSNRAIIRAPSVRGAAGVRGLGGRGYLAYTGLGRGYQVKGDKREDKLYDILPGMELTPMNPVTLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKITIPALASQNPAIHPFTPPKLSAFVDEAKTYAAEYTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNATAPVSAAQLKQAVTLGQDLAAYTTYEVYPTFAVTARGDGYGTF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (91)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
ACF MaxPab polyclonal antibody. Western Blot analysis of ACF expression in human placenta.Western Blot (Cell lysate)
A1CF MaxPab polyclonal antibody. Western Blot analysis of A1CF expression in HeLa.Western Blot (Transfected lysate)
Western Blot analysis of A1CF expression in transfected 293T cell line (H00029974-T02) by A1CF MaxPab polyclonal antibody.
Lane 1: ACF transfected lysate(65.34 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to A1CF on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — A1CF
Entrez GeneID
29974GeneBank Accession#
NM_138933Protein Accession#
NP_620311.1Gene Name
A1CF
Gene Alias
ACF, ACF64, ACF65, APOBEC1CF, ASP, MGC163391, RP11-564C4.2
Gene Description
APOBEC1 complementation factor
Gene Ontology
HyperlinkGene Summary
Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined. [provided by RefSeq
Other Designations
APOBEC-1 stimulating protein|OTTHUMP00000019611|OTTHUMP00000019612|OTTHUMP00000019614|OTTHUMP00000019615|OTTHUMP00000061209|apo-B RNA editing protein|apobec-1 complementation factor|apobec-1 complementation factor (ACF) (ASP)
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Interactome
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