ATG7 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human ATG7 full-length ORF ( NP_006386.1, 1 a.a. - 703 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MAAATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTADKKLLLEQAANEIWESIKSGTALENPVLLNKFLLLTFADLKKYHFYYWFCYPALCLPESLPLIQGPVGLDQRFSLKQIEALECAYDNLCQTEGVTALPYFLIKYDENMVLVSLLKHYSDFFQGQRTKITIGVYDPCNLAQYPGWPLRNFLVLAAHRWSSSFQSVEVVCFRDRTMQGARDVAHSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAADRLQKIFPGVNARGFNMSIPMPGHPVNFSSVTLEQARRDVEQLEQLIESHDVVFLLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDDETI
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
104.4
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — ATG7
Entrez GeneID
10533GeneBank Accession#
NM_006395.1Protein Accession#
NP_006386.1Gene Name
ATG7
Gene Alias
APG7-LIKE, APG7L, DKFZp434N0735, GSA7
Gene Description
ATG7 autophagy related 7 homolog (S. cerevisiae)
Omim ID
608760Gene Ontology
HyperlinkGene Summary
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq
Other Designations
APG7 autophagy 7-like|ubiquitin activating enzyme E1-like protein
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Interactome
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Pathway
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Disease
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Publication Reference
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A complex between Atg7 and caspase-9: a novel mechanism of cross-regulation between autophagy and apoptosis.
Han J, Hou W, Goldstein LA, Stolz DB, Watkins SC, Rabinowich H.
The Journal of Biological Chemistry 2014 Mar; 289(10):6485.
Application:Func, Recombinant protein.
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A complex between Atg7 and caspase-9: a novel mechanism of cross-regulation between autophagy and apoptosis.
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