CCNE2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CCNE2 transfected lysate.
Western Blot
Lane 1: CCNE2 transfected lysate ( 46.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CCNE2 full-length
Host
Human
Theoretical MW (kDa)
46.8
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CCNE2 antibody (H00009134-D01P) by Western Blots.
SDS-PAGE Gel
CCNE2 transfected lysate.
Western Blot
Lane 1: CCNE2 transfected lysate ( 46.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CCNE2
Entrez GeneID
9134GeneBank Accession#
NM_057749Protein Accession#
NP_477097.1Gene Name
CCNE2
Gene Alias
CYCE2
Gene Description
cyclin E2
Omim ID
603775Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq
Other Designations
G1/S-specific cyclin E2
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Interactome
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Pathway
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Disease
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