MUTYH monoclonal antibody (M01), clone 4D10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MUTYH.
Immunogen
MUTYH (AAH03178, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (70)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.
Lane 1: MUTYH transfected lysate(59.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MUTYH is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi ( Cat # H00004595-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody (M01), clone 4D10 (Cat # H00004595-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MUTYH
Entrez GeneID
4595GeneBank Accession#
BC003178Protein Accession#
AAH03178Gene Name
MUTYH
Gene Alias
MGC4416, MYH
Gene Description
mutY homolog (E. coli)
Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
A/G-specific adenine DNA glycosylase|OTTHUMP00000009096|OTTHUMP00000009098|OTTHUMP00000009101|OTTHUMP00000009102|mutY homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Oxidative guanine base damage plays a dual role in regulating productive ALT-associated homology-directed repair.
Sanjana A Thosar, Ryan P Barnes, Ariana Detwiler, Ragini Bhargava, Anne Wondisford, Roderick J O'Sullivan, Patricia L Opresko.
Cell Reports 2024 Jan; 43(1):113656.
Application:IF, Human, HeLa LT FAP, U2OS FAP cells.
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hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia.
Eshtad S, Mavajian Z, Rudd SG, Visnes T, Boström J, Altun M, Helleday T.
Oncogenesis 2016 Dec; 5(12):e275.
Application:WB, Human, HEK 293T, Jurkat cells.
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Reduced expression of the DNA glycosylase gene MUTYH is associated with an increased number of somatic mutations via a reduction in the DNA repair capacity in prostate adenocarcinoma.
Shinmura K, Kato H, Kawanishi Y, Yoshimura K, Igarashi H, Goto M, Tao H, Inoue Y, Sugiyama T, Furuse H, Ozono S, Sugimura H.
Molecular Carcinogenesis 2017 Feb; 56(2):781.
Application:WB-Tr, Human, PC-3 cells.
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Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia coli strain.
Komine K, Shimodaira H, Takao M, Soeda H, Zhang X, Takahashi M, Ishioka C.
Human Mutation 2015 Jul; 36(7):704.
Application:WB, Human, HCT116 cells.
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Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation.
Agustina L, Hahm SH, Han SH, Tran AH, Chung JH, Park JH, Park JW, Han YS.
BMC Molecular Biology 2014 Aug; 15(1):17.
Application:WB-Ce, Human, HEK293 cells.
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Impaired 8-Hydroxyguanine Repair Activity of MUTYH Variant p.Arg109Trp Found in a Japanese Patient with Early-Onset Colorectal Cancer.
Shinmura K, Goto M, Tao H, Kato H, Suzuki R, Nakamura S, Matsuda T, Yin G, Morita M, Kono S, Sugimura H.
Oxidative Medicine and Cellular Longevity 2014 Mar; 2014:617351.
Application:IF, WB-Tr, Human, H1299 cells.
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Human MutY homolog induces apoptosis in etoposide-treated HEK293 cells.
Hahm SH, Chung JH, Agustina L, Han SH, Yoon IS, Park JH, Kang LW, Park JW, Na JJ, Han YS.
Oncology Letters 2012 Dec; 4(6):1203.
Application:WB, Human, HEK 293 cells.
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Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells.
Raetz AG, Xie Y, Kundu S, Brinkmeyer MK, Chang C, David SS.
Carcinogenesis 2012 Nov; 33(11):2301.
Application:WB-Ce, Mouse , MEFs.
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Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer.
Shinmura K, Goto M, Suzuki M, Tao H, Yamada H, Igarashi H, Matsuura S, Maeda M, Konno H, Matsuda T, Sugimura H.
J Pathol 2011 Jun; 225:414.
Application:WB-Tr, Human, AGS cells.
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Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine.
Suzuki T, Harashima H, Kamiya H.
DNA Repair 2010 May; 9(5):542.
Application:WB-Tr, Human, HEK 293T cells.
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Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis.
van der Post RS, Kets CM, Ligtenberg MJ, van Krieken JH, Hoogerbrugge N.
Virchows Archiv 2009 Jan; 454(1):25.
Application:IHC-P, Human, Human adenoma cancer.
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Oxidative guanine base damage plays a dual role in regulating productive ALT-associated homology-directed repair.
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