HMGA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: HMGA1 transfected lysate ( 10.56 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
HMGA1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HMGA1 full-length
Host
Human
Theoretical MW (kDa)
10.67
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HMGA1 antibody (H00003159-B01) by Western Blots.
Western Blot
Lane 1: HMGA1 transfected lysate ( 10.56 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
HMGA1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HMGA1
Entrez GeneID
3159GeneBank Accession#
BC004924Protein Accession#
AAH04924Gene Name
HMGA1
Gene Alias
HMG-R, HMGA1A, HMGIY, MGC12816, MGC4242, MGC4854
Gene Description
high mobility group AT-hook 1
Omim ID
600701Gene Ontology
HyperlinkGene Summary
This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000016222|OTTHUMP00000016223|OTTHUMP00000016224|OTTHUMP00000039618|high-mobility group (nonhistone chromosomal) protein isoforms I and Y|nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y
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Interactome
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Disease
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