HK1 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Tissue lysate)
HK1 MaxPab polyclonal antibody. Western Blot analysis of HK1 expression in human spleen.
Western Blot (Transfected lysate)
Western Blot analysis of HK1 expression in transfected 293T cell line (H00003098-T01) by HK1 MaxPab polyclonal antibody.
Lane1:HK1 transfected lysate(100.76 KDa).
Lane2:Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of HK1 transfected lysate using anti-HK1 MaxPab mouse polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with HK1 purified MaxPab rabbit polyclonal antibody (D01P) (H00003098-D01P).
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to HK1 on HeLa cell. [antibody concentration 20 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human HK1 protein.
Immunogen
HK1 (NP_277031.1, 1 a.a. ~ 916 a.a) full-length human protein.
Sequence
MDCEHSLSLPCRGAEAWEIGIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTEASS
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
HK1 MaxPab polyclonal antibody. Western Blot analysis of HK1 expression in human spleen.Western Blot (Transfected lysate)
Western Blot analysis of HK1 expression in transfected 293T cell line (H00003098-T01) by HK1 MaxPab polyclonal antibody.
Lane1:HK1 transfected lysate(100.76 KDa).
Lane2:Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of HK1 transfected lysate using anti-HK1 MaxPab mouse polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with HK1 purified MaxPab rabbit polyclonal antibody (D01P) (H00003098-D01P).Immunofluorescence
Immunofluorescence of purified MaxPab antibody to HK1 on HeLa cell. [antibody concentration 20 ug/ml] -
Gene Info — HK1
Entrez GeneID
3098GeneBank Accession#
NM_033496.1Protein Accession#
NP_277031.1Gene Name
HK1
Gene Alias
HK1-ta, HK1-tb, HK1-tc, HKI, HXK1
Gene Description
hexokinase 1
Omim ID
142600Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq
Other Designations
OTTHUMP00000019725|brain form hexokinase|glycolytic enzyme
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Interactome
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Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
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