ENO1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ENO1 full-length
Host
Human
Theoretical MW (kDa)
47.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ENO1 antibody (H00002023-D01P) by Western Blots.
SDS-PAGE Gel
ENO1 transfected lysate.
Western Blot
Lane 1: ENO1 transfected lysate ( 47.20 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ENO1
Entrez GeneID
2023GeneBank Accession#
NM_001428.2Protein Accession#
NP_001419.1Gene Name
ENO1
Gene Alias
ENO1L1, MBP-1, MPB1, NNE, PPH
Gene Description
enolase 1, (alpha)
Omim ID
172430Gene Ontology
HyperlinkGene Summary
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq
Other Designations
2-phospho-D-glycerate hydro-lyase|MYC promoter-binding protein 1|OTTHUMP00000001706|alpha enolase like 1|enolase 1|non-neural enolase|phosphopyruvate hydratase|tau-crystallin
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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