BMPR1B monoclonal antibody (M01), clone 2F3
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of BMPR1B expression in transfected 293T cell line by BMPR1B monoclonal antibody (M01), clone 2F3.
Lane 1: BMPR1B transfected lysate(56.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BMPR1B is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of BMPR1B over-expressed 293 cell line, cotransfected with BMPR1B Validated Chimera RNAi ( Cat # H00000658-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BMPR1B monoclonal antibody (M01) clone 2F3 (Cat # H00000658-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (79.53 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant BMPR1B.
Immunogen
BMPR1B (AAH47773, 14 a.a. ~ 502 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KKEDGESTAPTPRPKVLRCKCHHHCPEDSVNNICSTDGYCFTMIEEDDSGLPVVTSGCLGLEGSDFQCRDTPIPHQRRSIECCTERNECNKDLHPTLPPLKNRDFVDGPIHHRALLISVTVCSLLLVLIILFCYFRYKRQETRPRYSIGLEQDETYIPPGESLRDLIEQSQSSGSGSGLPLLVQRTIAKQIQMVKQIGKGRYGEVWMGKWRGEKVAVKVFFTTEEASWFRETEIYQTVLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDYLKSTTLDAKSMLKLAYSSVSGLCHLHTEIFSTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVKFISDTNEVDIPPNTRVGTKRYMPPEVLDESLNRNHFQSYIMADMYSFGLILWEVARRCVSGGIVEEYQLPYHDLVPSDPSYEDMREIVCIKKLRPSFPNRWSSDECLRQMGKLMTECWAHNPASRLTALRVKKTFAKMSESQDIKL
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (79.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of BMPR1B expression in transfected 293T cell line by BMPR1B monoclonal antibody (M01), clone 2F3.
Lane 1: BMPR1B transfected lysate(56.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BMPR1B is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of BMPR1B over-expressed 293 cell line, cotransfected with BMPR1B Validated Chimera RNAi ( Cat # H00000658-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BMPR1B monoclonal antibody (M01) clone 2F3 (Cat # H00000658-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — BMPR1B
Entrez GeneID
658GeneBank Accession#
BC047773Protein Accession#
AAH47773Gene Name
BMPR1B
Gene Alias
ALK-6, ALK6, CDw293
Gene Description
bone morphogenetic protein receptor, type IB
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. [provided by RefSeq
Other Designations
OTTHUMP00000161622|activin receptor-like kinase 6|serine/threonine receptor kinase
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Interactome
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