ACTN1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ACTN1 transfected lysate.
Western Blot
Lane 1: ACTN1 transfected lysate ( 103.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACTN1 full-length
Host
Human
Theoretical MW (kDa)
103.1
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACTN1 antibody (H00000087-D01P) by Western Blots.
SDS-PAGE Gel
ACTN1 transfected lysate.
Western Blot
Lane 1: ACTN1 transfected lysate ( 103.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACTN1
Entrez GeneID
87GeneBank Accession#
NM_001102.2Protein Accession#
NP_001093.1Gene Name
ACTN1
Gene Alias
FLJ40884
Gene Description
actinin, alpha 1
Omim ID
102575Gene Ontology
HyperlinkGene Summary
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
F-actin cross-linking protein|actinin 1 smooth muscle|alpha-actinin 1
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Interactome
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Pathway
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