This antibody pair set comes with matched antibody pair to detect and quantify protein level of human ACTN4.
Quality Control Testing:
Standard curve using recombinant protein ( H00000081-Q01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 1 ng/ml to 100 ng/ml.
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-ACTN4, IgG2a (100 ug) 2. Detection antibody: biotinylated mouse monoclonal anti-ACTN4, IgG2a Kappa (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq