ACAT2 monoclonal antibody (M01), clone 4A5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ACAT2.
Immunogen
ACAT2 (AAH00408, 1 a.a. ~ 397 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNAGSDPVVIVSAARTIIGSFNGALAAVPVQDLGSTVIKEVLKRATVAPEDVSEVIFGHVLAAGCGQNPVRQASVGAGIPYSVPAWSCQMICGSGLKAVCLAVQSIGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEMPLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRRGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPANASGINDGAAAVVLMKKSEADKRGLTPLARIVSWSQVGVEPSIMGIGPIPAIKQAVTKAGWSLEDVDIFEINEAFAAVSAAIVKELGLNPEKVNIEGGAIALGHPLGASGCRILVTLLHTLERMGRSRGVAALCIGGGMGIAMCVQRE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (87); Rat (84)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (69.41 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ACAT2 expression in transfected 293T cell line by ACAT2 monoclonal antibody (M01), clone 4A5.
Lane 1: ACAT2 transfected lysate(41.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACAT2 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — ACAT2
Entrez GeneID
39GeneBank Accession#
BC000408Protein Accession#
AAH00408Gene Name
ACAT2
Gene Alias
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Gene Description
acetyl-Coenzyme A acetyltransferase 2
Omim ID
100678Gene Ontology
HyperlinkGene Summary
The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq
Other Designations
OTTHUMP00000017527|acetoacetyl Coenzyme A thiolase|cytosolic acetoacetyl-CoA thiolase
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Interactome
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Pathway
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Disease
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