제품

Product Description

Human ADAM8 (P78325, Ile17-Pro655) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Sequence

Ile17-Pro655

Host

Human

Theoretical MW (kDa)

70.90000000000001

Form

Lyophilized

Preparation Method

Mammalian cell (HEK293) expression system

Purity

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Endotoxin Level

< 1 EU per 1 ug of protein (determined by LAL method)

Activity

The EC₅₀ was 8.1 ng/mL, messured by ELISA at 0.5 ug/mL. Measured by the ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is > 1 pmol/min/ug.

Quality Control Testing

SEC-HPLC and Tris-Bis PAGE

SEC-HPLC

The purity of Human ADAM8 is greater than 95% as determined by SEC-HPLC.

Tris-Bis PAGE

Human ADAM8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Recommend Usage

Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from sterile distilled Water is > 100 ug/mL

Storage Instruction

Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis