
VHH-His tag NanoAb™ Targeting Human TNFRSF1A

* The price is valid only in USA. Please select country.
- More Functions
-
Specifications
Product Description
VHH NanoAb™ targeting TNFRSF1A expressed in Escherichia coli under conditions free from animal-derived components.
Antibody Species
Alpaca
Theoretical MW (kDa)
14.2
Reactivity
Human
Epitope
Monospecific
Tag
His tag at C-terminus
Specificity
Tumor necrosis factor receptor 1 (TNFR1) / CD120a
Preparation Method
Escherichia coli expression system
Purification
Affinity chromatography purified
Conjugation
Unconjugated
Affinity
Not measured
Form
Lyophilized
Purity
> 95% by SDS-PAGE
Recommend Usage
ELISA (1:1000-1:10000)
WB (1:500-1:3000)
IHC (1:100-1:300)
IF (1:100-1:300)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS pH 7.4.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water or with 50% glycerol (ACS grade or better), store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Lysates of human hepatocellular carcinoma tissue were repectively subjected to SDS-PAGE followed by western blot with TNFRSF1A VHH recombinant monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.Western Blot
Lysates of A549 cells were repectively subjected to SDS-PAGE followed by western blot with TNFRSF1A VHH recombinant monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.Immunohistochemistry
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue slides using TNFRSF1A VHH recombinant monoclonal antibody at 2.5 ug/mL and positive control at 1:200 dilution (under 20x lens), respectively. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed MCF-7 cells stained with TNFRSF1A VHH recombinant monoclonal antibody at 2.5 ug/mL followed by CoraLite® 488 secondary antibody at 1:200 dilution, showing cytoplasmic staining (under 40x lens). The nuclear stain is DAPI (blue).Enzyme-linked Immunoabsorbent Assay
Indirect ELISA showing TNFRSF1A VHH recombinant monoclonal antibody binding to purified PD-L1. Plates were coated with 200 ng/well purified protein and binding of TNFRSF1A VHH recombinant monoclonal antibody assessed in serial dilution from 1 ng/mL primary antibody in triplicate.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com