VHH-His tag NanoAb™ Targeting Human TNFRSF1A

Catalog # NAB00008

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Price

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Quantity

Size:100 ug
Price: USD $ 329.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot
Application

Western Blot

Lysates of human hepatocellular carcinoma tissue were repectively subjected to SDS-PAGE followed by western blot with TNFRSF1A VHH recombinant monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Western Blot
Application

Western Blot

Lysates of A549 cells were repectively subjected to SDS-PAGE followed by western blot with TNFRSF1A VHH recombinant monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemistry
Application

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue slides using TNFRSF1A VHH recombinant monoclonal antibody at 2.5 ug/mL and positive control at 1:200 dilution (under 20x lens), respectively. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescence
Application

Immunofluorescence

Immunofluorescence analysis of paraformaldehyde fixed MCF-7 cells stained with TNFRSF1A VHH recombinant monoclonal antibody at 2.5 ug/mL followed by CoraLite® 488 secondary antibody at 1:200 dilution, showing cytoplasmic staining (under 40x lens). The nuclear stain is DAPI (blue).

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

Indirect ELISA showing TNFRSF1A VHH recombinant monoclonal antibody binding to purified PD-L1. Plates were coated with 200 ng/well purified protein and binding of TNFRSF1A VHH recombinant monoclonal antibody assessed in serial dilution from 1 ng/mL primary antibody in triplicate.

  • Specifications

    Product Description

    VHH NanoAb™ targeting TNFRSF1A expressed in Escherichia coli under conditions free from animal-derived components.Nanobody,Nanobodies,sdAb,VHH,sdAbs,VHHs,Nano Antibody,Nano Antibodies,NanoAb,Nano Ab,NanoAbTM,MAB,monoclonal Ab,monoclonal antibody

    Antibody Species

    Alpaca

    Theoretical MW (kDa)

    14.2

    Reactivity

    Human

    Epitope

    Monospecific

    Tag

    His tag at C-terminus

    Specificity

    Tumor necrosis factor receptor 1 (TNFR1) / CD120a

    Preparation Method

    Escherichia coli expression system

    Purification

    Affinity chromatography purified

    Conjugation

    Unconjugated

    Affinity

    Not measured

    Form

    Lyophilized

    Purity

    > 95% by SDS-PAGE

    Recommend Usage

    ELISA (1:1000-1:10000)
    WB (1:500-1:3000)
    IHC (1:100-1:300)
    IF (1:100-1:300)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from PBS pH 7.4.

    Storage Instruction

    Store at 4°C on dry atmosphere.
    After reconstitution with deionized water or with 50% glycerol (ACS grade or better), store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

    Lysates of human hepatocellular carcinoma tissue were repectively subjected to SDS-PAGE followed by western blot with TNFRSF1A VHH recombinant monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

    Western Blot

    Lysates of A549 cells were repectively subjected to SDS-PAGE followed by western blot with TNFRSF1A VHH recombinant monoclonal antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue slides using TNFRSF1A VHH recombinant monoclonal antibody at 2.5 ug/mL and positive control at 1:200 dilution (under 20x lens), respectively. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunofluorescence

    Immunofluorescence analysis of paraformaldehyde fixed MCF-7 cells stained with TNFRSF1A VHH recombinant monoclonal antibody at 2.5 ug/mL followed by CoraLite® 488 secondary antibody at 1:200 dilution, showing cytoplasmic staining (under 40x lens). The nuclear stain is DAPI (blue).

    Enzyme-linked Immunoabsorbent Assay

    Indirect ELISA showing TNFRSF1A VHH recombinant monoclonal antibody binding to purified PD-L1. Plates were coated with 200 ng/well purified protein and binding of TNFRSF1A VHH recombinant monoclonal antibody assessed in serial dilution from 1 ng/mL primary antibody in triplicate.
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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