
VHH-His tag NanoAb™ Targeting Human CD274

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Specifications
Product Description
VHH NanoAb™ targeting CD274 expressed in Escherichia coli under conditions free from animal-derived components.
Antibody Species
Alpaca
Theoretical MW (kDa)
14.4
Reactivity
Crab-eating macaque, Human
Epitope
Monospecific
Tag
His tag at C-terminus
Specificity
Human PD-L1
Preparation Method
Escherichia coli expression system
Purification
Affinity chromatography
Conjugation
Unconjugated
Affinity
Not measured
Form
Lyophilized
Purity
> 95% by SDS-PAGE
Recommend Usage
ELISA (1:10000-1:100000)
WB (1:1000-1:3000)
IHC (1:200-1:800)
IF (1:200-1:800)
FC (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS pH 7.4.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water or with 50% glycerol (ACS grade or better), store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Lysates (30 ug) were subjected to SDS-PAGE followed by western blot with CD274 VHH recombinant monoclonal antibody at dilution of 1:1000 incubated overnight at 4°C.Immunohistochemistry
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slides using CD274 VHH recombinant monoclonal antibody at 2.5ug/ml and positive control at 1:500 dilution (under 40x lens), respectively. Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).Immunohistochemistry
Immunohistochemical analysis of paraffin-embeddedhuman lung cancer tissue slides using CD274 VHH recombinant monoclonal antibody at 2.5ug/ml and positive control at 1:500 dilution (under 40x lens), respectively. Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed SH-SY5Y cells stained with CD274 VHH recombinant monoclonal antibody at 2 ug/mL. The nuclear stain is DAPI (blue).Enzyme-linked Immunoabsorbent Assay
Indirect ELISA showing CD274 VHH recombinant monoclonal antibody binding to purified PD-L1. Plates were coated with 200 ng/well-purified protein and the binding of CD274 VHH recombinant monoclonal antibody was assessed in serial dilution from 0.06 ng/mL primary antibody in triplicate.Flow Cytometry
PD-L1 stable expressing PLC/PRF/5 cells were stained with CD274 VHH recombinant monoclonal antibody at a dilution of 1:50.Surface Plasmon Resonance
Captured human PD-L1 on CM5 Chip can bind CD274 VHH recombinant monoclonal antibody with an affinity constant of 0.363 nM as determined in SPR assay (Biacore T200).
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