Camelid single domain antibody (sdAb), also known as VHH and nanobody, is the recombinant variable domain derived from camelid heavy-chain-only antibody. This configuration confers a number of unique properties. The small size and high stability of sdAb make it a promising tool for disease diagnosis and treatment. sdAb can penetrate tissue more easily and target difficult epitopes hidden in protein structure. Also, sdAb expresses well in many host systems and it is cost-effective to manufacture on a large scale.
Abnova offers Camelid Single Domain Antibody Service for production of target-specific sdAb from naïve camelid VHH library. By using the advanced naïve camelid VHH library screening technology, we screen the library and isolate the sdAb clones for your required antigens. Positive clones are sequenced and validated by ELISA. The selected clones will then be introduced into HEK293 expression vector or E. coli expression vector for transient and scalable sdAb production for many downstream applications.
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ELISA Results of VHH Clone Isolation |
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ELISA results of selected phage clones after 6 rounds of panning | |
ELISA Results of Selected Clones as Recombinant Anti-CD8 Bivalent VHH hIgG1 LALAPG Ab |
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ELISA QC of selected clones Recombinant Anti-CD8 Bivalent VHH hIgG1 LALAPG Ab | |
For any inquiry, please contact : OEM@abnova.com