VIM (Phospho S56) recombinant monoclonal antibody

Catalog # RAB02555

Size

Price

Stock

Quantity

Size:100 uL
Price: USD $ 531.00
Stock:
order now, ship in 5 days
abnova-minus
abnova-plus

* The price is valid only in USA. Please select country.

Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis of U251MG (A) whole cell lysates with VIM (Phospho S56) recombinant monoclonal antibody (Cat # RAB02555).

Immunofluorescence
Application

Immunofluorescence

Immunofluorescent analysis of HeLa cells with VIM (Phospho S56) recombinant monoclonal antibody (Cat # RAB02555). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against human VIM.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs,VIM,Vimentin,CSV

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser56 of human VIM.

    Theoretical MW (kDa)

    53

    Reactivity

    Human

    Specificity

    Recognizes endogenous levels of Vimentin (pS56) protein.

    Form

    Liquid

    Purification

    Immunogen affinity chromatography

    Isotype

    IgG

    Recommend Usage

    Immunocytochemistry (1:50-1:100)
    Immunofluorescence (1:50-1:100)
    Immunoprecipitation (1:10-1:50)
    Western Blot (1:500-1:1000)

    Storage Buffer

    In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)

    Storage Instruction

    Store at 4°C short term.
    Aliquot and store at -20°C long term.
    Avoid freeze-thaw cycles.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western blot analysis of U251MG (A) whole cell lysates with VIM (Phospho S56) recombinant monoclonal antibody (Cat # RAB02555).

    Immunocytochemistry

    Immunofluorescence

    Immunofluorescent analysis of HeLa cells with VIM (Phospho S56) recombinant monoclonal antibody (Cat # RAB02555). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

    Immunoprecipitation

  • Gene Info — VIM

    Entrez GeneID

    7431

    Protein Accession#

    P08670

    Gene Name

    VIM

    Gene Alias

    FLJ36605

    Gene Description

    vimentin

    Omim ID

    193060

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract

    Other Designations

    OTTHUMP00000019224

  • Interactomes
  • Diseases
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
4 Products to Compare
Remove All