SELE (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human E-Selectin, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 20.5 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human E-selectin on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
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Specifications
Product Description
Human SELE (P16581, Trp22-Pro556) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Trp22-Pro556
Host
Human
Theoretical MW (kDa)
85.40000000000001
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 20.5 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human E-selectin on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human E-Selectin, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 20.5 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — SELE
Entrez GeneID
6401Protein Accession#
P16581Gene Name
SELE
Gene Alias
CD62E, ELAM, ELAM1, ESEL, LECAM2
Gene Description
selectin E
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq
Other Designations
OTTHUMP00000032745|endothelial adhesion molecule 1|leukocyte endothelial cell adhesion molecule 2
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Interactomes
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Pathways
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Diseases
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