PVR (Human) Recombinant Protein (Biotin)
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human CD155, His Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 10.6 ng/mL determined by ELISA.
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human CD155, His Tag at 1 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 3.0 ng/mL determined by ELISA (QC Test).
Tris-Bis PAGE
Biotinylated Human PVR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human PVR is greater than 95% as determined by SEC-HPLC.
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Specifications
Product Description
Human PVR (P15151-1, Trp21-Asn343) partial recombinant protein with His-Avi tag at the C-Terminus expressed in HEK293 cells.
Sequence
Trp21-Asn343
Host
Human
Theoretical MW (kDa)
38
Reactivity
Human
Form
Lyophilized
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by Tris-Bis PAGE
> 95% by HPLCEndotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Activity
The EC50 was 3.0 ng/mL, measured by ELISA at 1 ug/mL.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
SEC-HPLC
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. (8% trehalose).
Storage Instruction
Store at -20°C for 12 months.
After reconstitution, store at 4°C for 2-7 days, or store at -80°C for 3-6 months.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human CD155, His Tag at 1 ug/mL (100 uL/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 10.6 ng/mL determined by ELISA.Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human CD155, His Tag at 1 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 3.0 ng/mL determined by ELISA (QC Test).Functional Study
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Gene Info — PVR
Entrez GeneID
5817Protein Accession#
P15151-1Gene Name
PVR
Gene Alias
CD155, FLJ25946, HVED, NECL5, Necl-5, PVS, TAGE4
Gene Description
poliovirus receptor
Omim ID
173850Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
nectin-like 5
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Interactomes
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Pathways
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Diseases
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