APOE (Human) Recombinant Protein (Biotin)
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More Files
- More Functions
Surface Plasmon Resonance
Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human APOE3, His Tag with an affinity constant of 1.41 uM as determined in SPR assay (Biacore T200).
Tris-Bis PAGE
Biotinylated Human APOE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Specifications
Product Description
Human APOE (P02649-1, Lys19-His317) partial recombinant protein with His tag at the N-Terminus expressed in HEK293 cells.
Sequence
Lys19-His317
Host
Human
Theoretical MW (kDa)
35.3
Reactivity
Human
Form
Lyophilized
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by Tris-Bis PAGE
Endotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Activity
The affinity constant was 1.41 uM, determined by SPR assay.
Recommend Usage
Biological Activity
Tris-Bis PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. (8% trehalose).
Storage Instruction
Store at -20°C for 12 months.
After reconstitution, store at 4°C for 2-7 days, or store at -80°C for 3-6 months.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
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Applications
Functional Study
Surface Plasmon Resonance
Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human APOE3, His Tag with an affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). -
Gene Info — APOE
Entrez GeneID
348Protein Accession#
P02649-1Gene Name
APOE
Gene Alias
AD2, LPG, MGC1571, apoprotein
Gene Description
apolipoprotein E
Gene Ontology
HyperlinkGene Summary
Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq
Other Designations
apolipoprotein E3
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Interactomes
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Diseases
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