ACE2 (Human) Recombinant Protein
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More Files
- More Functions
Surface Plasmon Resonance
SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).
ELISA
Immobilized Human ACE2, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4 ng/ml determined by ELISA (QC Test).
SEC-HPLC
The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC
Tris-Bis PAGE
Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specifications
Product Description
Human ACE2 (Q9BYF1-1, Gln18-Ser740) partial recombinant protein with His-Avi tag at C-terminus expressed in HEK293 cells.
Sequence
Gln18-Ser740
Host
Human
Theoretical MW (kDa)
86.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
ELISA and SEC-HPLC
ELISA
Immobilized Human ACE2, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 7.4 ng/ml determined by ELISA (QC Test).
SEC-HPLC
The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC
Tris-Bis PAGE
Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SEC-HPLC
SPR
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (5% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Functional Study
Surface Plasmon Resonance
SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). -
Gene Info — ACE2
Entrez GeneID
59272Protein Accession#
Q9BYF1-1Gene Name
ACE2
Gene Alias
ACEH, DKFZp434A014
Gene Description
angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Omim ID
300335Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq
Other Designations
ACE-related carboxypeptidase|OTTHUMP00000022963|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein
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Interactomes
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Pathways
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Diseases
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