ACE2 (Human) Recombinant Protein

Catalog # P10023

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Price

Stock

Quantity

Size:100 ug
Price: USD $ 351.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Surface Plasmon Resonance
Application

Surface Plasmon Resonance

SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9 nM as determined in a SPR assay (Biacore T200).

ELISA
QC Test

ELISA

Immobilized Human ACE2, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 46.2 ng/ml determined by ELISA (QC Test).

SEC-HPLC
QC Test

SEC-HPLC

The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC

Tris-Bis PAGE
QC Test

Tris-Bis PAGE

Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

  • Specifications

    Product Description

    Human ACE2 (Q9BYF1-1, Gln18-Ser740) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

    Sequence

    Gln18-Ser740

    Host

    Human

    Theoretical MW (kDa)

    84.7

    Form

    Lyophilized

    Preparation Method

    Mammalian cell (HEK293) expression system

    Purity

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    Endotoxin Level

    < 1 EU per 1 ug of protein (determined by LAL method)

    Quality Control Testing

    ELISA and SEC-HPLC

    ELISA

    Immobilized Human ACE2, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 46.2 ng/ml determined by ELISA (QC Test).

    SEC-HPLC

    The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC

    Tris-Bis PAGE

    Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

    Recommend Usage

    Biological Activity
    ELISA
    SEC-HPLC
    SPR
    Tris-Bis PAGE
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from filtered solution in PBS, pH 7.4 (8% trehalose).

    Storage Instruction

    After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Functional Study

    Surface Plasmon Resonance

    SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9 nM as determined in a SPR assay (Biacore T200).
  • Gene Info — ACE2

    Entrez GeneID

    59272

    Protein Accession#

    Q9BYF1-1

    Gene Name

    ACE2

    Gene Alias

    ACEH, DKFZp434A014

    Gene Description

    angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

    Omim ID

    300335

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq

    Other Designations

    ACE-related carboxypeptidase|OTTHUMP00000022963|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein

  • Interactomes
  • Pathways
  • Diseases
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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