COL3A1 monoclonal antibody, clone FH-7A
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of whole cell lysates with COL3A1 monoclonal antibody, clone COL-29 (Cat # MAB1514).
Lane 1 : HT1080
Lane 2 : Colo 320
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Specifications
Product Description
Mouse monoclonal antibody raised against native COL3A1.
Immunogen
Native purified human COL3A1.
Host
Mouse
Theoretical MW (kDa)
70
Reactivity
Human, Mouse, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Western Blot (0.25-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Immunohistochemistry (Frozen sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 1.2% sodium acetate (2 mg BSA, 0.01 mg sodium azide)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of whole cell lysates with COL3A1 monoclonal antibody, clone COL-29 (Cat # MAB1514).
Lane 1 : HT1080
Lane 2 : Colo 320Immunohistochemistry
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Gene Info — COL3A1
Entrez GeneID
1281Gene Name
COL3A1
Gene Alias
EDS4A, FLJ34534
Gene Description
collagen, type III, alpha 1
Gene Ontology
HyperlinkGene Summary
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish
Other Designations
Ehlers-Danlos syndrome type IV, autosomal dominant|alpha1 (III) collagen|collagen type III alpha 1|collagen, fetal
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Interactomes
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Pathways
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Diseases
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Publication Reference
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A Novel Systems-Biology Algorithm for the Analysis of Coordinated Protein Responses Using Quantitative Proteomics.
Garcia-Marques F, Trevisan-Herraz M, Martinez-Martinez S, Camafeita E, Jorge I, Lopez JA, Mendez-Barbero N, Mendez-Ferrer S, Del Pozo MA, Ibanez B, Andrrs V, Ssnchez-Madrid F, Redondo JM, Bonzon-Kulichenko E, Vszquez J.
Molecular & Cellular Proteomics 2016 May; 15(5):1740.
Application：IF, Mouse, Primary mouse vascular smooth muscle cells.
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Less Contribution of Mast Cells to the Progression of Renal Fibrosis in Rat Kidneys with Chronic Renal Failure.
Baba A, Tachi M, Ejima Y, Endo Y, Toyama H, Saito K, Yamauchi M, Miura C, Kazama I.
Nephrology 2017 Feb; 22(2):159.
Application：IHC-P, Rat, Kidneys.
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Mast cell involvement in the progression of peritoneal fibrosis in rats with chronic renal failure.
Kazama I, Baba A, Endo Y, Toyama H, Ejima Y, Matsubara M, Tachi M.
Nephrology 2015 Sep; 20(9):609.
Application：IHC, Rat, Peritoneum.
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Benidipine suppresses in situ proliferation of leukocytes and slows the progression of renal fibrosis in rat kidneys with advanced chronic renal failure.
Kazama I, Baba A, Matsubara M, Endo Y, Toyama H, Ejima Y.
Nephron Experimental Nephrology 2014 Oct; 128(1-2):67.
Application：IHC, Rat, Kidney.
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Overexpression of Delayed Rectifier K(+) Channels Promotes In situ Proliferation of Leukocytes in Rat Kidneys with Advanced Chronic Renal Failure.
Kazama I, Maruyama Y, Endo Y, Toyama H, Ejima Y, Matsubara M, Kurosawa S.
International Journal of Nephrology 2012 May; 2012:581581.
Application：IHC-P, Rat, Rat kidneys.
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Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.
Liu X, Wu H, Byrne M, Krane S, Jaenisch R.
PNAS 1997 Mar; 94(5):1852.
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Physical mapping by PFGE localizes the COL3A1 and COL5A2 genes to a 35-kb region on human chromosome 2.
Cutting GR, McGinniss MJ, Kasch LM, Tsipouras P, Antonarakis SE.
Genomics 1990 Oct; 8(2):407.
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A Novel Systems-Biology Algorithm for the Analysis of Coordinated Protein Responses Using Quantitative Proteomics.
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