PTK2 monoclonal antibody (M01), clone 2C3

Catalog # H00005747-M01

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Size:100 ug
Price: USD $ 335.00
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  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
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Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

PTK2 monoclonal antibody (M01), clone 2C3 Western Blot analysis of PTK2 expression in Hela S3 NE ( Cat # L013V3 ).

Immunoprecipitation
Application

Immunoprecipitation

Immunoprecipitation of PTK2 transfected lysate using anti-PTK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTK2 MaxPab rabbit polyclonal antibody.

Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged PTK2 is approximately 0.03ng/ml as a capture antibody.

<em>In situ</em> Proximity Ligation Assay (Cell)
Application

In situ Proximity Ligation Assay (Cell)

Proximity Ligation Analysis of protein-protein interactions between STAT1 and PTK2. HeLa cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-PTK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

QC Test

Western Blot detection against Immunogen (40.59 KDa) .

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against a partial recombinant PTK2.

    Immunogen

    PTK2 (AAH28733, 355 a.a. ~ 490 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    EGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQ

    Host

    Mouse

    Reactivity

    Human

    Isotype

    IgG1 Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Western Blot detection against Immunogen (40.59 KDa) .

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Cell lysate)

    PTK2 monoclonal antibody (M01), clone 2C3 Western Blot analysis of PTK2 expression in Hela S3 NE ( Cat # L013V3 ).

    Western Blot (Recombinant protein)

    Immunoprecipitation

    Immunoprecipitation of PTK2 transfected lysate using anti-PTK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTK2 MaxPab rabbit polyclonal antibody.

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged PTK2 is approximately 0.03ng/ml as a capture antibody.

    ELISA

    In situ Proximity Ligation Assay (Cell)

    Proximity Ligation Analysis of protein-protein interactions between STAT1 and PTK2. HeLa cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-PTK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Gene Info — PTK2

    Entrez GeneID

    5747

    GeneBank Accession#

    BC028733

    Protein Accession#

    AAH28733

    Gene Name

    PTK2

    Gene Alias

    FADK, FAK, FAK1, pp125FAK

    Gene Description

    PTK2 protein tyrosine kinase 2

    Omim ID

    600758

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq

    Other Designations

    focal adhesion kinase 1

  • Interactomes
  • Pathways
  • Diseases
  • Publication Reference
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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