GCLM monoclonal antibody (M01), clone 2B8
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More Files
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Western Blot (Cell lysate)
GCLM monoclonal antibody (M01), clone 2B8 Western Blot analysis of GCLM expression in HeLa ( Cat # L013V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GCLM is approximately 1ng/ml as a capture antibody.
Western Blot detection against Immunogen (55.88 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GCLM.
Immunogen
GCLM (AAH41809, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSGASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.88 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GCLM monoclonal antibody (M01), clone 2B8 Western Blot analysis of GCLM expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GCLM is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — GCLM
Entrez GeneID
2730GeneBank Accession#
BC041809Protein Accession#
AAH41809Gene Name
GCLM
Gene Alias
GLCLR
Gene Description
glutamate-cysteine ligase, modifier subunit
Omim ID
601176Gene Ontology
HyperlinkGene Summary
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq
Other Designations
GSC light chain|OTTHUMP00000012299|gamma-glutamylcysteine synthetase|glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD)|glutamate-cysteine ligase regulatory protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Protection against 2-chloroethyl ethyl sulfide (CEES) - Induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.
Abel EL, Bubel JD, Simper MS, Powell L, McClellan SA, Andreeff M, Macleod MC, Digiovanni J.
Toxicol Appl Pharmacol 2011 Jun; 255:176.
Application：WB-Ce, Human, NCTC 2544 cells.
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Molecular and Functional Characterization of Gastrula Organizer Cells Derived from Human Embryonic Stem Cells.
Sharon N, Mor I, Golan-Lev T, Fainsod A, Benvenisty N.
Stem Cells 2011 Apr; 29(4):600.
Application：IF, Human, Embryoid bodies.
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Diversity in Antioxidant Response Enzymes in Progressive Stages of Human Nonalcoholic Fatty Liver Disease.
Hardwick RN, Fisher CD, Canet MJ, Lake AD, Cherrington NJ.
Drug Metabolism and Disposition 2010 Dec; 38(12):2293.
Application：WB-Ti, Human , Human liver samples.
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Induction of Antioxidant Enzymes by Curcumin and Its Analogues in Human Islets: Implications in Transplantation.
Balamurugan AN, Akhov L, Selvaraj G, Pugazhenthi S.
Pancreas 2009 May; 38(4):454.
Application：WB-Ti, Human, Human islets.
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Protection against 2-chloroethyl ethyl sulfide (CEES) - Induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.
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