BTLA (Human) Recombinant Protein (Biotin)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HVEM, hFc Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 1.4ug/mL determined by ELISA.
Enzyme-linked Immunoabsorbent Assay
Immobilized Anti-BTLA Antibody, hFc Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0 ng/mL determined by ELISA (QC Test).
Tris-Bis PAGE
Biotinylated Human BTLA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC
-
Specifications
Product Description
Human BTLA (Q7Z6A9-1, Lys31-Ser150) partial recombinant protein with His-Avi tag at the C-Terminus expressed in HEK293 cells.
Sequence
Lys31-Ser150
Host
Human
Theoretical MW (kDa)
16.7
Reactivity
Human
Form
Lyophilized
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by Tris-Bis PAGE
> 95% by HPLCEndotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Activity
The EC50 was 8.0 ng/mL, measured by ELISA at 0.5 ug/mL.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
SEC-HPLC
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. (8% trehalose).
Storage Instruction
Store at -20°C for 12 months.
After reconstitution, store at 4°C for 2-7 days, or store at -80°C for 3-6 months.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HVEM, hFc Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 1.4ug/mL determined by ELISA.Enzyme-linked Immunoabsorbent Assay
Immobilized Anti-BTLA Antibody, hFc Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0 ng/mL determined by ELISA (QC Test).Functional Study
-
Gene Info — BTLA
Entrez GeneID
151888Protein Accession#
Q7Z6A9-1Gene Name
BTLA
Gene Alias
BTLA1, CD272, FLJ16065, MGC129743
Gene Description
B and T lymphocyte associated
Omim ID
607925Gene Ontology
HyperlinkGene Summary
BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM
Other Designations
B and T lymphocyte attenuator
-
Interactomes
-
Diseases
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com