AXL (Human) Recombinant Protein (Biotin)
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Anti-Axl Antibody at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 26.8 ng/mL determined by ELISA.
Enzyme-linked Immunoabsorbent Assay
Immobilized Human GAS6, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 9.2 ng/mL determined by ELISA (QC Test).
Tris-Bis PAGE
Biotinylated Human AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human AXL is greater than 95% as determined by SEC-HPLC.
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Specifications
Product Description
Human AXL (P30530-1, Ala26-Pro449) partial recombinant protein with hFc tag at the C-Terminus expressed in HEK293 cells.
Sequence
Ala26-Pro449
Host
Human
Theoretical MW (kDa)
72.59999999999999
Reactivity
Human
Form
Lyophilized
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by Tris-Bis PAGE
> 95% by HPLCEndotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Activity
The EC50 was 26.8 ng/mL, measured by ELISA at 0.5 ug/mL.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
SEC-HPLC
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. (8% trehalose).
Storage Instruction
Store at -20°C for 12 months.
After reconstitution, store at 4°C for 2-7 days, or store at -80°C for 3-6 months.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Anti-Axl Antibody at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 26.8 ng/mL determined by ELISA.Enzyme-linked Immunoabsorbent Assay
Immobilized Human GAS6, His Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 9.2 ng/mL determined by ELISA (QC Test).Functional Study
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Gene Info — AXL
Entrez GeneID
558Protein Accession#
P30530-1Gene Name
AXL
Gene Alias
JTK11, UFO
Gene Description
AXL receptor tyrosine kinase
Omim ID
109135Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
AXL transforming sequence/gene|oncogene AXL
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Interactomes
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Diseases
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